微生物降解1,2,4-三氯苯研究进展

1,2,4-三氯苯(1,2,4-TCB)的微生物降解机制包括好氧降解、还原脱氯和共代谢。本文简要综述了国内外对1,2,4-TCB微生物降解方面的研究进展,分析了其微生物降解的可行性,讨论了其好氧降解的微生物种类以及降解途径,阐述了厌氧条件下,混合菌群以及纯菌株对1,2,4-TCB还原脱氯的过程,目的是为1,2,4-TCB污染土壤的微生物修复提供依据。     

Occurrence of Polycyclic Musks in Sewage Sludge and their Behaviour in Soils and Plants. Part 1: Behaviour of Polycyclic Musks in Sewage Sludge of Different Treatment Plants in Summer and Winter (5 pp)

Background, Aim and Scope   Part 1: Behaviour of Polycyclic Musks in Sewage Sludge of Different Treatment Plants in Summer and Winter Part 2: Investigation of Polycyclic Musks in Soils and Plants -  Preamble. In Part 1 of the study, screening tests were performed to investigate the occurrence of PCMs in sewage sludges. For a preliminary risk assessment, further information is needed about their behaviour in the terrestrial environment. Hence, Part 2 examined the adsorption of PCMs to soil, their dissipation and leaching in soil and their uptake by plants. Background, Aim and Scope   Polycyclic Musks (PCMs) enter the environment via the waste water system. Because of their persistence, they can accumulate in different matrices like sewage sludge or biota. By the use of sewage sludge as a fertilizer, PCMs are transferred to agricultural soils. Therefore, in Part 1 of the study, screening tests were performed to investigate the occurrence of PCMs in sewage sludge. For a preliminary risk assessment, further information is needed about their behaviour in the terrestrial environment. Hence, Part 2 of the study examined the adsorption of PCMs to soil, their dissipation and leaching in soil, and their uptake by plants. Materials and Methods: In the screening study, samples of activated sewage sludge were taken both in summer and in winter at 21 treatment plants. In order to get an overview of the contamination situation, sampling covered different types of treatment plants (in rural, urban, industrial areas). Analytical methods for the determination of HHCB, AHTN, ADBI, ATTN, AHDI and ATII in the sludge samples were developed and applied. Results: The analytical screening of PCMs showed their presence in activated and dried sewage sludge samples. HHCB and AHTN represented about 95% of the PCMs investigated. Their concentrations in the activated sludge samples varied between 2.9 and 10.4 mg/kg dry mass (dm) and 1.1 to 4.2 mg/kg dm, respectively. Although different types of sewage treatment plants were investigated, similar PCM levels were found, showing the widespread input of these compounds into domestic waste water. Discussion: PCM concentrations in activated sludge varied widely. The variation drops substantially when concentrations are related to the varying dry mass. In dehydrated sludge, PCM concentrations were up to 24 mg/kg dm for HHCB and up to 6.9 mg/kg dm for AHTN. These high values are comparable to those obtained in other investigations analysing PCMs. If the degradation of organic mass during anaerobic decomposition is included in the evaluation, the figures obtained are comparable to those for activated sludge. Elimination in sewage sludge was higher in summer than in winter. Therefore, the contamination of the sludges in winter reached higher levels compared to the summer. Conclusions: The results show that PCMs are widespread contaminants in sewage sludge. Recommendations and Perspectives: PCM should be considered in a risk assessment as potential contaminants of sewage sludge destined for agricultural use. Due to the high PCM levels in sewage sludge, further investigations into the degradation and elimination behaviour in sewage sludge have to be carried out, including that involving PCM metabolites such as lactone derivatives.     

Defining the validity of a biochemical index of soil quality

 The native soils of Galicia (NW Spain) exhibit a biochemical equilibrium such that total soil N is a function of five biochemical and microbiological parameters: microbial biomass C, mineralized N, phosphomonoesterase, β-glucosidase and urease activities. To investigate whether the ratio of the total N calculated from biochemical soil properties (Nc) and the total N as measured by the Kjeldahl method (Nk; Nc/Nk) can be used as an index of soil quality, we determined these variables and consequently the ratio in three kinds of disturbed soils: an artificially Cu-contaminated soil, two lignite mine soils, and a number of arable soils. In none of the studied soils did the individual biochemical parameters respond consistently to the factors influencing soil quality, but in all cases soil degradation was reflected by the Nc/Nk value, which differed more or less markedly from 100%. Nc/Nk can therefore be used for the rapid evaluation of soil degradation, since it distinguishes among biochemically balanced soils, soils in a transient state of high microbiological and biochemical activity and degraded soils. It can also serve as a reliable basis for the rapid calculation of the "ecological dose" (ED₅₀) of soil pollutants. The use of Nc/Nk as an objective index of the biochemical quality of soils is recommended. Received: 20 December 1998     

Fractal features of soil particle size distribution and the implication for indicating desertification

Some of the effects of land desertification on soil properties are manifested by the coarsening of the soil particlesize distribution (PSD) and the losses in organic C and nutrients. The changes and characteristics of PSD and selected chemical properties in soils at the 0-15 cm plough layer from different degrees of desertified croplands are analyzed in the semiarid Horqin Sandy Land, northern China. The fractal dimension of the PSD is emanated to characterize the patterns of PSD. The relationships between the fractal dimension of the PSD and selected soil properties are discussed. The results show that: (1) in the transformation from potential desertified cropland to extremely desertified cropland, the sand content at the 0-15 cm soil increased from 69% to 93%, organic C and total N contents decreased by 65% and 69%, respectively; (2) the fractal dimension of PSD ranged from 2.179 to 2.611, the more the contents of sand, the lower the fractal dimension and the higher the desertified degree of farmland. In the desertification process within the studied area, the mean fractal dimension decreased from 2.555 for the potential desertified soils to 2.298 for extremely desertified soils; (3) there existed considerable linear relationships between fractal dimension and soil properties. It was shown that fractal dimensions of PSD are useful parameters able to monitor soil degradation and to estimate the degree of soil desertification.     

淹水土壤中久效磷的降解及消失动态

本试验在实验室模拟土壤淹水条件下,研究微生物对久效磷残留及降解的影响。结果表明:在未经灭菌的土壤中,久效磷及其降解物的消失比在灭菌土壤中快,相应的回归方程为C_WT=5.4502e~(-0.0254)t,C_YT=5.8509—0.0423t,残留半衰期分别为27.3和69.2天。久效磷在淹水土壤中降解为甲醇可抽提态和不可抽提态残留物,对甲醇可抽提态残留物经TLC和红外光谱分析表明,其降解物为磷酸三酯、N-去甲基久效磷、O,O-二甲基乙烯基磷酸酯,0-去甲基久效磷,以及O,C-去甲基久效磷,但主要为磷酸三酯,其含量由母液中的5％增加到28—83％,这种降解过程,在未灭菌土壤中比在灭菌土壤中快。     

低丘红壤旱地水分问题及其解决途径研究进展

阐述了低丘红壤旱地的季节性干旱和土壤物理性退化等导致的水分问题 ,总结了一些实际可行的减缓水分问题的有效措施 ,如 :农林复合生态系统的构建、植被覆盖、合理轮作、以肥调水、灌溉系统和改良土壤结构等。并在回顾有关研究的基础上 ,对低丘红壤区水分问题的研究和解决途径提出展望     

Symbiosis with bacteria enhances the use of chitin by the springtail,Folsomia candida (Collembola)

Summary The relationship between Folsomia candida and chitin-degrading microorganisms was studied. On chitin agar, 10¹⁰ bacteria were isolated per g faeces, and 3.8×10¹¹ bacteria per g gut contents, 1/3 of them showing a clear (chitin-free) zone around the colony. The most abundant chitin-degrading bacteria were Xanthomonas maltophilia and Curtobacterium sp. To determine the bacterial contribution in the use of chitin by F. candida, a feeding experiment was carried out. F. candida were fed with chitin, either amended with or without tetracycline as an inhibitor of bacteria. When tetracycline was omitted the biomass of F. candida was increased compared to those fed chitin with tetracycline. However, this result was observed only when the food replacement intervals were long enough to allow bacterial colonization before ingestion of the food. In a food-selection experiment, a preference for chitin colonized with microorganisms as opposed to sterile chitin was found. The results indicate that a mutualistic symbiosis of F. candida with chitinolytic microorganisms is likely to enhance chitin degradation. This relationship is not only intra-intestinal but also involves an extra-intestinal phase.Dedicated to the late Prof. Dr. W. Kühnelt     

Landsliding related to land-cover change: A diachronic analysis of hillslope instability distribution in the Sierra Norte,Puebla, Mexico

This study examines mass movement associated with land use change, particularly deforestation, from multiple perspectives. The significance of such understanding is related to the degree of impact landsliding may cause on human settlements and economic activities, and on forest ecosystems. In this paper, the distribution of hillslope instability in the Sierra Norte, Puebla, Mexico is addressed by means of a diachronic analysis, which involves the development of vegetation indexes, as well as vegetation fragmentation derived from Landsat-5 (TM) and Landsat-7 (ETM+) satellite images from 1989 and 1999, respectively. The time period was chosen to compare vegetation cover conditions prior and after the extreme October 1999 rainfall event that triggered hundreds of slope failures in the study area. Results suggested there was a significant vegetation reduction from 1989 to 1999, which was strongly expressed by an increase of 809 km² of bare surfaces. Additionally, areas with highest vegetation density (91–100%) decreased considerably, from 1245 to 363 km², resulting in a net vegetation reduction of 70%. Furthermore, it was possible to highlight that landslide concentration was much higher on surfaces that were bare and had low vegetation density (0–50%), representing 85% of hillslope instability, than on surfaces having a greater density of vegetation cover. Land use change and land degradation are precursors to environmental hazards, such as mass movement events, that pose serious threats to regional population distributions and economic vitality.     

Impacts of the ancient Maya on soils and soil erosion in the central Maya Lowlands

Many studies across the central and southern Maya Lowlands of Belize, Guatemala, Honduras, and Mexico have produced records of land degradation, mostly sedimentation and soil erosion, during the ancient Maya period from before 1000 BC to the Maya Collapse of c. AD 900. This paper provides new data from two sites (Blue Creek and Cancuén), synthesizes more than a decade of the authors' research in Guatemala, Belize, and Mexico, and synthesizes other findings from this region. These research projects analyzed more than 100 excavations in upland and depression sites, cored lakes and wetland sediments, and studied sediments in the field and laboratory using radiocarbon dating, a battery of soil chemistry tests, stratigraphic analysis, magnetic susceptibility, elemental analyses, and artifact identification. Our objective was to date when sedimentation and soil erosion occurred, identify stable surfaces, and correlate them with the state of knowledge about past land use. These findings indicate three general epochs of accelerated soil erosion and identified two major paleosols. The three waves of soil erosion occurred in the Preclassic period (c. 1000 BC to AD 250), the Late Classic (AD 550 to 900), and in the last several decades. The major paleosol (‘Eklu'um’) in these sites is a well-developed Mollisol or Vertisol that started forming in the early Holocene and was buried in either the Preclassic or Classic periods (AD 250 to 900). At some sites the Eklu'um paleosol lies beneath sediments with a fainter paleosol, which in turn lies buried below Classic period and later sediments. This picture shows higher than expected soil erosion linked to the region's first pioneer farmers in the Preclassic and less than expected soil erosion in the Late Classic when population peaked and land use was the most intensive. In other regions like Cancuén, Guatemala, however, most soil erosion occurred during the Maya Late Classic (AD 550–830). Erosion here was intense but short-lived: depressions record 1–3 m of aggradation in two centuries. A third epoch of accelerated soil loss and aggradation arose with the rapid land use changes brought by new pioneers during the last several decades.